01-04 May, 2017 – Theoretical lectures
01.05.2017, Monday
Auditorium E-207, Ilia State University, 3/5 Cholokashvili Avenue, Tbilisi
9.00 – 10:30 – Registration
10:30 – Opening of Seasonal School
11:00 – 12:00 – Lecture 1. “A Voyage Through Time & Space: Atomic Force Microscopy, and the birth of NanoCellBiology”, Professor Bhanu Jena
12:00 – 12:15 – Coffee Break
12:15 – 13:15 – Lecture 2. “Historical overview of the Secretion & Membrane Fusion”, Professor Bhanu Jena
13:15 – 14:15 – Lunch
14:15 – 15:15 – Lecture 3. “New Cellular Structures at the Plasma Membrane of Exocrine Pancreas are ‘Secretory Portals’ or ‘Porosomes’ “, Professor Bhanu Jena
15:15 – 15:30 – Coffee Break
15:30 – 16:30 – Lecture 4. “ The Ultrastructure of Synapses”, Associate Professor Tamar Bikashvili
02.05.2017, Tuesday
Auditorium E-207, Ilia State University, 3/5 Cholokashvili Avenue, Tbilisi
10:00 – 11:00 – “Composition and Functional Reconstitution of ‘Porosomes’ in Artificial Lipid Membrane”, Professor Bhanu Jena
11:00 – 11:15 – Coffee Break
11:15 – 12:15 – “Porosomes in Neurons and in Endocrine Cells”, Professor Bhanu Jena
12:15 – 12:30 – Coffee Break
12:30 – 13:30 – Lecture 3. “Porosome in Cystic Fibrosis”, Professor Bhanu Jena
13:30 – 14:30 – Lunch
14:30 – 15:30 – Lecture 4. “The Ultrastructure of Neurons, Synapses and Glial Cells and Neurological Disorders”, Professor Ia Zhvania
15:30 – 15:45 – Coffee Break
15:45 – 16:45 – Lecture 5. “Membrane –Associated SNAREs and their Involvement in Calcium-Mediated Membrane Fusion”, Professor Bhanu Jena
03.05.2017, Wednesday
Auditorium E-207, Ilia State University, 3/5 Cholokashvili Avenue, Tbilisi
10:00 – 11:00 – Lecture 1. “Role of Lipids on the Structure and Assembly of SNAREs”, Professor Bhanu Jena
11:00 – 11:15 – Coffee Break
11:15 – 12:15 – Lecture 2. “Secretory Vesicles Volume Increase is a Requirement for the Regulated for Expulsion of Intravesicular Contents from Cells During Cell Secretion”, Professor Bhanu Jena
12:15 – 12:30 – Coffee Break
12:30 – 13:30 – Lecture 3. “The Molecular Mechanisms of Secretory Vesicle Volume Regulation in Cells”, Professor Bhanu Jena
13:30 – 14:30 – Lunch
14:30 – 15:30 – Lecture 4. „Role of pH on Cell Secretion”, Professor Bhanu Jena
15:30 – 15:45 – Coffee Break
15:45 – 16:45 – Lecture 5. “The Ultrastructure of Neuronal Porosomal Complex in Norm and Pathologies”, Professor Nana Japaridze
04.05.2017, Thursday
Auditorium E-207, Ilia State University, 3/5 Cholokashvili Avenue, Tbilisi
11:00 – 12:00 – Lecture 1. “Biogenesis of Biological Membranes”, Professor Bhanu Jena
12:00 – 12:15 – Coffee Break
12:15 – 13:15 – Lecture 2. “Single Molecular Thermometry” – Professor Bhanu Jena
13:15 – 14:15 – Lunch
14:15 – 15:15 – Lecture 3. “Ultra-rapid Detection of Antibiotic Resistant Bacteria by Atomic Force Microscopy” – Dr. Sandor Kasas
15:15 – 15:30 – Coffee Break
15:30 – 16:30 – Lecture 4. “The Neuron-Glia Interactions – Light Microscopic and Electron Microscopic level” – Associate Professor Tamar Lordkipanidze
5.05. 2017 – 9.05 .2017: Practical Courses on Electron Microscopy (EM) and Atomic Force Microscopy (AFM).
Students will attend EM and AFM studies in two parallel groups: “A” and “B”.
05.05.2017, Friday
Group “A” – EM Studies
Laboratory of Brain Ultrastructure and Nanoarchitecture, Ivane Beritashvili Center of Exprerimental Biomedicine, Fourth Floor, 14 Gotua Street, Tbilisi
10:00 – 11:00 – Welcome, Introduction to EM studies
11:00 – 17:00 – Brain tissue sample preparation for EM studies (Perfusion of experimental animals, brain tissue preparation, processing, embedding)
13:00 – 14:00 – Lunch
Lecturers: Giorgi Lobzhanidze, Nino Pochkhidze, Lia Gelazonia
Group “B” – AFM Studies
Laboratory of Brain Ultrastructure and Nanoarchitecture, Ivane Beritashvili Center of Exprerimental Biomedicine, Fourth Floor, 14 Gotua Street
10:00 – 11:00 – Main Principles of AFM Studies
11:00 – 17.00 – AFM Session
13:00 – 14:00 – Lunch
Lecturers: David Khutsishvili, Micheil Mebonia, Vera Okuneva, Ia Zhvania
05. 2017, Saturday
Group “A” – EM Studies
Laboratory of Brain Ultrastructure and Nanoarchitecture, Ivane Beritashvili Center of Exprerimental Biomedicine, Fourth Floor ,14 Gotua Street, Tbilisi
10:00 – 17:00 – Preparation of brain tissue samples for EM observation (Preparation of diamond knifes and cooper grids; Preparation of semithin, thin and ultrathin brain sections; Staining of brain sections).
13:00 – 14:00 – Lunch
Lecturers: Tamar Lordkipanidze, Nino Pochkhidze, Nato Kotaria
Group “B” – AFM Studies
10:00 – 17:00 – AFM Session
13:00 – 14:00 – Lunch
Lecturers: David Khutsishvili, Micheil Mebonia, Vera Okuneva, Ia Zhvania
07.05. 2017, Sunday
Group “A” – EM Studies
George Eliava Institute of Bacteriophages, Microbiology and Virology, 3 Gotua Street
10:00 – 17:00 – EM Session
13:00 – 14:00 – Lunch
Lecturers: Giorgi Tsertsvadze, Ia Zhvania
Group “B” – EM Studies
Laboratory of Brain Ultrastructure and Nanoarchitecture, Ivane Beritashvili Center of Exprerimental Biomedicine, Fourth Floor ,14 Gotua Street, Tbilisi
10:00 – 11:00 – Welcome. Introduction to EM studies
11:00 – 17:00 – Brain tissue sample preparation for EM studies (Perfusion of experimental animals, brain tissue preparation, processing, embedding)
13:00 – 14:00 – Lunch
Lecturers: Giorgi Lobzhanidze, Nino Pochkhidze, Lia Gelazonia
08.05. 2017, Monday
Group “A” – AFM Studies
Laboratory of Brain Ultrastructure and Nanoarchitecture, Ivane Beritashvili Center of Exprerimental Biomedicine, Fourth Floor ,14 Gotua Street
10:00 – 12:00 – Main Principles of AFM
13:00 – 14:00 – Lunch
14:00 – 17:00 – AFM Studies
Lecturers: David Khutsishvili, Micheil Mebonia, Vera Okuneva, Nana Japaridze
Group “B” – EM Studies
Laboratory of Brain Ultrastructure and Nanoarchitecture, Ivane Beritashvili Center of Exprerimental Biomedicine, Fourth Floor ,14 Gotua Street
10:00 – 17:00 – Preparation of brain tissue samples for EM observation (Preparation of diamond knifes and cooper grids; Preparation of semithin, thin and ultrathin brain sections; Staining of brain sections).
13:00 – 14:00 – Lunch
Lecturers: Tamar Lordkipanidze, Nino Pochkhidze, Nato Kotaria
09.05. 2017. Tuesday
Group “A” – AFM Studies
Laboratory of Brain Ultrastructure and Nanoarchitecture, Ivane Beritashvili Center of Experimental Biomedicine, Fourth Floor, 14 Gotua Street
10:00 – 17:00 – AFM Session
13:00 – 14:00 – Lunch
Lecturers: DaviT Khutsishvili, Micheil Mebonia, Vera Okuneva, Nana Japaridze
Group “B” – EM Studies
Giorgi Eliava Institute of Bacteriophages, Microbiology and Virology, 3 Gotua Street
10:00 – 17:00 – EM Session
13:00 – 14:00 – Lunch
Lecturers: Giorgi Cercvadze, Ia Zhvania
17:00 – Closing of Seasonal School – Ivane Beritashvili Center of Experimental Biomedicine, 14 Gotua Street, Tbilisi